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Junior Research Group Terrestrial Biofilms | Drucken |

Forschung

Bakterielle Populationen können sich durch die Entwicklung unterschiedlichster Überlebensstrategien gut an widrige Umweltbedingungen anpassen. Der gram-positive Modellorganismus Bacillus subtilis stellt ein hervorragendes Beispiel dafür dar, wie phenotypische Heterogeneität die verschiedenen Entwicklungswege beeinflusst. Die Bildung von
B. subtilis Biofilmen setzt komplexe regulatorische Mechanismen voraus, ist abhängig von Zell-Zell Kommunikation und involviert heterogene Genexpression. Das Ziel unserer Arbeitsgruppe ist es, einen tieferen Einblick in die Rolle von Wachstumsstrategien in Biofilmen (Konkurrenz zwischen Wachstumsrate und -ertrag) zu gewinnen. Weiterhin beschäftigen wir uns mit Interaktionen zwischen verschiedenen Arten von Bakterien oder zwischen Bakterien und Pilzen und die Auswirkungen von Schwermetallen auf Biofilmentwicklung und -differenzierung. Fluoreszenzmikroskopische Methoden und mathematische Modelle werden kombiniert um ein besseres Verständnis der Differenzierung komplexer Biofilmpopulationen zu ermöglichen.

 

Weitere Informationen sind auf der Webseite der Gruppe "Terrestrische Biofilme" zu finden:

https://sites.google.com/site/terrestrialbiofilms/

 

Mitarbeiter

Dr. Ákos T. Kovács, Nachwuchsgruppenleiter
(Büro E004, tel: 3641-9-49980)

Anna Dragos, Postdoc (Alexander von Humboldt Fellow)
(Büro E006, tel: 3641-9-49984)

Eisha Mhatre, Doktorandin (JSMC)
(Büro E005, tel: 3641-9-49983)

Ramses Gallegos Monterrosa, Doktorand (CONACyT-DAAD)
(Büro E005, tel: 3641-9-49983)

Theresa Hölscher, Doktorandin (IMPRS)
(Büro E005, tel: 3641-9-49983)

Marivic Martin, Doktorandin (DFG)
(Büro E005, tel: 3641-9-49983)

Anne Richter, Doktorandin (DFG)
(Büro E006, tel: 3641-9-49984)

Tina Schiklang, Masterstudentin
(Büro E005, tel: 3641-9-49983)

Daniel Schäfer, Masterstudent
(Büro E005, tel: 3641-9-49983)

Valliyammai Ramaswamhi, Masterstudentin
(Büro E006, tel: 3641-9-49984)

Surabhi, Masterstudentin (2015-2016)/Hiwi (2016-2017)
(Büro E006, tel: 3641-9-49984)

Heiko Kiesewalter, Masterstudent (2016)/Hiwi (2017)
(Büro K008)

 

 

Alumni:

Tino Barchewitz, Masterstudent (2015-2016)
Anandaroopan Sundaram, Masterstudent (2015-2016)
Tadeja Marin, MSc Gaststudentin (2016)
Felix Blei, Masterstudent (2014-2015), Bachelorstudent (2013)
Tim Sehrt, Masterstudent (2014-2015)
Benjamin Bartels, Masterstudent (2014)
Manasa Bharathwaj, Bachelorstudentin (2015)
Jan Schwitalla, Bachelorstudent (2015)
Sonja Koppenhöfer, Bachelorstudentin (2015)
Stefanie Lindstädt, Bachelorstudentin (2015)
Wieland Schrewe, Bachelorstudent (2014)
Stefanie Kankel, Bachelorstudentin (2014)
Heiko Kiesewalter, Bachelorstudent (2013)

 

Lebenslauf des Nachwuchsgruppenleiters

ab Juli 2017 Professur für Bakterielle Physiologie und Genetik, Technical University Denmark, Dänemark

10/2012-06/2017 Nachwuchsgruppenleiter, Institut für Mikrobiologie, FSU Jena, Deutschland

2014 Mitglied der International Max Planck Research School (IMPRS)

2013 Mitglied der Jena School for Microbial Communication (JSMC)

2005-2012 Promovierter wissenschaftlicher Mitarbeiter, Universität Groningen, die Niederlande

2003-2005 Promovierter wissenschaftlicher Mitarbeiter, Biological Research Center, Szeged, Ungarn

2003 Promotion, Universität Szeged, Ungarn

2000 Diplom in Biologie, Universität Szeged, Ungarn

 

PUBLIKATIONEN

(PDFs: https://sites.google.com/site/terrestrialbiofilms/ )

2017

Dragoš A, Kovács ÁT (2017) The peculiar functions of bacterial extracellular matrix. Trends in Microbiology in press doi: 10.1016/j.tim.2016.12.010

Krawinkel J, Torres-Mapa ML, Mhatre E, Kovács ÁT, Heisterkamp A (2017) Structural damage of Bacillus subtilis biofilms using pulsed laser interaction with gold thin films. Journal of Biophotonics in press doi: 10.1002/jbio.201600146

van den Esker M, Kovács ÁT, Kuipers OP (2016) YsbA and LytST are essential for pyruvate utilization in Bacillus subtilis. Environmental Microbiology in press doi: 10.1111/1462-2920.13454

Raie D*, Mhatre E*, Thiele M, Labena AE, Al-Ghannam GA, Farahat LA, Youssef T, Fritzsche W, Kovács ÁT (2017) Application of quercetin and its bio-inspired nanoparticles as anti-adhesive agents against Bacillus subtilis attachment to surface. Materials Science and Engineering C 70:753-762 doi: 10.1016/j.msec.2016.09.038

2016

Gallegos-Monterrosa R, Maróti G, Bálint B, Kovács ÁT (2016) Draft genome sequence of the soil isolate Lysinibacillus fusiformis M5, a potential hypoxanthine producer. Genome Announcements 4(6):e01272-16 doi: 10.1128/genomeA.01272-16

Gallegos-Monterrosa R, Mhatre E, Kovács ÁT (2016) Specific Bacillus subtilis 168 variants do form biofilms on nutrient rich medium. Microbiology 162(11):1922-1932 doi: 10.1099/mic.0.000371

Seccareccia I, Kovács ÁT, Gallegos-Monterrosa R, Nett M (2016) Unraveling the predator-prey relationship of Cupriavidus necator and Bacillus subtilis. Microbiological Research 192:231-238

Mhatre E, Troszok A, Gallegos-Monterrosa R, Lindstädt S, Hölscher T, Kuipers OP, Kovács ÁT (2016) The impact of manganese on biofilm development of Bacillus subtilis. Microbiology 162(8):1468-1478 doi: 10.1099/mic.0.000320

Hölscher T, Dragoš A, Gallegos-Monterrosa R, Martin M, Mhatre E, Richter A, Kovács ÁT (2016) Monitoring spatial segregation in surface colonizing microbial populations. Journal of Visualized Experiments 116:e54752, doi: 10.3791/54752

Kovács ÁT (2016) The global regulator CodY is required for the fitness of Bacillus cereus in various laboratory media and certain beverages. FEMS Microbiology Letters 363(13): fnw216 doi: 10.1093/femsle/fnw126

Martin M*, Hölscher T*, Dragoš A*, Cooper VS, Kovács ÁT (2016) Laboratory evolution of microbial interactions in bacterial biofilms. Journal of Bacteriology 198(19):2564-2571 doi: 10.1128/JB.01018-15 *contributed equally

Kovács ÁT (2016) Bacterial differentiation via gradual activation of global regulators. Current Genetics 62(1): 125-128. doi: 10.1007/s00294-015-0524-8

2015

Hölscher T*, Bartels B*, Lin Y-C*, Gallegos-Monterrosa R, Price-Whelan A, Kolter R, Dietrich LEP, Kovács ÁT (2015) Motility, chemotaxis and aerotaxis contribute to competitiveness during bacterial pellicle biofilm development. Journal of Molecular Biology 427(23): 3695-3708. doi: 10.1016/j.jmb.2015.06.014 *contributed equally

Kovács ÁT (2015) Bacterial differentiation via gradual activation of global regulators. Current Genetics in press 10.1007/s00294-015-0524-8

Grau R, de Oña P, Kunert M, Leñini C, Gallegos-Monterrosa R, Mhatre E, Vileta D, Donato V, Hölscher T, Boland W, Kuipers OP, Kovács ÁT (2015) A duo of potassium-responsive histidine kinases govern the multicellular destiny of Bacillus subtilis. mBio 6(3):e00581-15. doi:10.1128/mBio.00581-15

Benoit I, van den Esker MH, Patyshakuliyeva A, Mattern D, Blei F, Zhou M, Dijksterhuis J, Brakhage AA, Kuipers OP, de Vries RP, Kovács ÁT (2015) Bacillus subtilis attachment to Aspergillus niger hyphae results in mutually altered metabolism. Environmental Microbiology 17(6): 2099-2113. doi: 10.1111/1462-2920.12564

Hölscher T, Kost C, Kovács ÁT (2015) Einblicke in das Sozialleben von Mikroben. Biospektrum 21(3):264-266. doi: 10.1007/s12268-015-0569-8

Schneider J, Klein T, Mielich-Süss B, Koch G, Franke C, Kuipers OP, Kovács ÁT, Sauer M, Lopez D (2015) Spatio-temporal remodeling of functional membrane microdomains organizes the signaling networks of a bacterium. PLoS Genetics 11(4):e1005140 doi: 10.1371/journal.pgen.1005140

Detert Oude Weme R, Kovács ÁT, de Jong S, Veening JW, Siebring J, Kuipers OP (2015) Single cell FRET analysis for the identification of optimal FRET-pairs in Bacillus subtilis using a prototype MEM-FLIM system. PLoS One 10(4):e0123239 doi: 10.1371/journal.pone.0123239

2014

van Gestel J, Weissing FJ, Kuipers OP, Kovács ÁT (2014) Density of founder cells affects spatial pattern formation and cooperation in Bacillus subtilis biofilms. ISME Journal 8(10): 2069–2079. doi: 10.1038/ismej.2014.52

Kovács ÁT (2014) Impact of spatial distribution on the development of mutualism in microbes. Frontiers in Microbiology 5: 649. doi:10.3389/fmicb.2014.00649

Mhatre E*, Gallegos Monterrosa R*, Kovács ÁT (2014) From environmental signals to regulators: modulation of biofilm development in Gram-positive bacteria. Journal of Basic Microbiology 54(7): 616-632. *contributed equally

Kovács ÁT, Kothe E (2014) Special issue: Biofilms. Journal of Basic Microbiology 54(7): 615 (Editorial)

Puentes-Téllez PE, Kovács ÁT, Kuipers OP, van Elsas JD (2014) Comparative genomics and transcriptomics analysis of experimentally evolved Escherichia coli MC1000 in complex environments. Environmental Microbiology 16(3): 856-870.

Irigul-Sonmez O, Koroglu TE, Ozturk B, Kovács ÁT, Kuipers OP, Yazgan-Karatas A (2014) In Bacillus subtilis LutR is part of the global complex regulatory network governing the adaptation to the transition from exponential growth to stationary phase. Microbiology 160(2): 243-260.

Diethmaier C, Newman JA, Kovács ÁT, Kaever V, Herzberg C, Rodrigues C, Boonstra M, Kuipers OP, Lewis RJ, Stülke J (2014) The YmdB phosphodiesterase is a global regulator of late adaptive responses in Bacillus subtilis. Journal of Bacteriology 196(2): 265-275.

Siebring J, Elema MJH, Drubi Vega F, Kovács ÁT, Haccou P, Kuipers OP (2014) Repeated triggering of sporulation in Bacillus subtilis selects against a protein that affects timing of cell division. ISME Journal 8(1): 77-87.

2013

Overkamp W, Beilharz K, Detert Oude Weme R, Solopova A, Karsens H, Kovács ÁT, Kok J, Kuipers OP, Veening JW (2013) Benchmarking various GFP variants in Bacillus subtilis, Streptococcus pneumoniae and Lactococcus lactis for live cell imaging. Applied and Environmental Microbiology 79(20): 6481-6490.

Lehnik-Habrink M, Rempeters L, Kovács ÁT, Wrede C, Baierlein C, Krebber H, Kuipers OP, Stülke J (2013) The DEAD-box RNA helicases in Bacillus subtilis have multiple functions and act independent from each other. Journal of Bacteriology 195(3): 534-544.

Kovács ÁT, Eckhardt TH, van Kranenburg R, Kuipers OP (2013) Functional analysis of the ComK protein of Bacillus coagulans. PLOS One 8(1): e53471.

2012

Kovács ÁT, van Gestel J, Kuipers OP (2012) The protective layer of biofilm: a repellent function for a new class of amphiphilic proteins. Molecular Microbiology 85(1):8-11.

Fibriansah G, Kovács ÁT, Pool TJ, Boonstra M, Kuipers OP, Thunnissen AMWH (2012) Crystal structures of two transcriptional regulators from Bacillus cereus define the conserved structural features of a PadR subfamily. PLOS One 7(11): e48015.

Lindbäck T, Mols M, Basset C, Granum PE, Kuipers OP, Kovács ÁT (2012) CodY, a pleiotropic regulator, influences multicellular behavior and efficient production of virulence factors in Bacillus cereus. Environmental Microbiology 14(8):2233-2246.

2011

Mironczuk AM, Manu A, Kuipers OP, Kovács ÁT (2011) Distinct roles of ComK1 and ComK2 in gene regulation in Bacillus cereus. PLOS One 6(7): e21859

Mellegård H, Kovács ÁT, Lindbäck T, Christensen BE, Kuipers OP, Granum PE (2011) Transcriptional responses of Bacillus cereus towards challenges with the polysaccharide chitosan. PLOS One 6(9):e24304

Kovács ÁT, Kuipers OP (2011) Rok regulates yuaB expression during architecturally complex colony development of Bacillus subtilis 168. Journal of Bacteriology 193(4): 998-1002 Cover illustration of the issue of February 2011

Abee T, Kovács ÁT, Kuipers OP, van der Veen S (2011) Biofilm formation and dispersal in Gram-positive bacteria. Current Opinion in Biotechnology 22(2): 172-179 Cover illustration of the issue of April 2011

2010

Kovács ÁT, van Hartskamp M, Kuipers OP, van Kranenburg R (2010) Genetic tool development for a new host for biotechnology, the thermotolerant bacterium Bacillus coagulans. Applied and Environmental Microbiology 76(12): 4085-4088

2009

Kovács ÁT, Smits WK, Mironczuk AM, Kuipers OP (2009) Ubiquitous late competence genes in Bacillus species indicate the presence of functional DNA uptake machineries. Environmental Microbiology 11(8): 1911-1922

Grande Burgos MJ*, Kovács ÁT*, Mironczuk AM, Abriouel H, Galvez A, Kuipers OP (2009) Response of Bacillus cereus ATCC 14579 to challenges with sublethal concentrations of enterocin AS-48. BMC Microbiology 9:227 *contributed equally

2008 und früher

Mironczuk AM, Kovács ÁT, Kuipers OP (2008) Induction of natural competence in Bacillus cereus ATCC14579. Microbial Biotechnology 1(3): 226-235.

Kovács ÁT, Rákhely G, Balogh J, Maróti G, Cournac L, Carrier P, Mészáros LS, Peltier G, Kovács KL (2005) Hydrogen independent expression of hupSL genes in Thiocapsa roseopersicina. FEBS Journal 272(18): 4807-4816.

Kovács ÁT, Rákhely G, Kovács KL (2005) The PpsR regulator family. (Mini-review) Research in Microbiology 156(5-6): 619-625.

Kovács ÁT, Rákhely G, Browning DF, Fülöp A, Maróti G, Busby SJW, Kovács KL (2005) An FNR-type regulator controls the anaerobic expression of Hyn hydrogenase in Thiocapsa roseopersicina. Journal of Bacteriology 187(8): 2618-2627.

Kovács ÁT, Rákhely G, Balogh J, Maróti G, Fülöp A, Kovács KL (2005) Anaerobic regulation of hydrogenase transcription in different bacteria. (Mini-review) Biochemical Society Transactions 33(1): 36-38.

Kovács KL, Kovács ÁT, Maróti G, Mészáros LS, Balogh J, Latinovics D, Fülöp A, Dávid R, Dorogházi E, Rákhely G (2005) The hydrogenases of Thiocapsa roseopersicina. Biochemical Society Transactions 33(1): 61-63.

Kovács KL, Kovács ÁT, Maróti G, Bagi Z, Csanádi G, Perei K, Bálint B, Balogh J, Fülöp A, Mészáros LS, Tóth A, Dávid R, Latinovics D, Varga A, Rákhely G (2005) Improvement of biohydrogen production and intensification of biogas formation. Reviews in Environmental Science and Biotechnology 3(4): 321-330.

Rákhely G, Kovács ÁT, Maróti G, Fodor BD, Csanádi G, Latinovics D, Kovács KL (2004) A cyanobacterial type, heteropentameric NAD+ reducing [NiFe] hydrogenase in the purple sulfur photosynthetic bacterium, Thiocapsa roseopersicina. Applied and Environmental Microbiology 64(2): 722-728. (IF 3.8)

Fodor BD, Kovács ÁT, Csáki R, Hunyadi-Gulyás E, Klement E, Maróti G, Mészáros LS, Medzihradszky KF, Rákhely G, Kovács KL (2004) Modular broad-host-range expression vectors for single protein and protein complex purification. Applied and Environmental Microbiology 64(2): 712-721.

Kovács ÁT, Rákhely G, Kovács KL (2003) Genes involved in the biosynthesis of photosynthetic pigments in the purple sulfur photosynthetic bacterium Thiocapsa roseopersicina. Applied and Environmental Microbiology 63(6): 3093-3102.

Maróti G, Fodor BD, Rákhely G, Kovács ÁT, Arvani S, Kovács KL (2003) Accessory proteins functioning selectively and pleiotropically in the biosynthesis of [NiFe] hydrogenases in Thiocapsa roseopersicina. European Journal of Biochemistry 270(10):2218-2227.

Kovács KL, Fodor B, Kovács ÁT, Csanádi G, Maróti G, Balogh J, Arvani S, Rákhely G (2002) Hydrogenases, accessory genes and the regulation of [NiFe] hydrogenase biosynthesis in Thiocapsa roseopersicina. International Journal of Hydrogen Energy 27:1463-1469.

Fodor B, Rákhely G, Kovács ÁT, Kovács KL (2001) Transposon mutagenesis in purple sulfur photosynthetic bacteria: identification of hypF, encoding a protein capable of processing [NiFe] hydrogenases in alpha, beta, and gamma subdivisions of the proteobacteria. Applied and Environmental Microbiology 61(6): 2476-2483.

Dahl C, Rákhely G, Pott AS, Fodor B, Takács M, Tóth A, Kraeling M, Győrfi K, Kovács Á, Tusz J, Kovács KL (1999) Genes involved in hydrogen and sulfur metabolism in phototrophic sulfur bacteria. FEMS Microbiology Letters 180(2):317-324.

Buchkapitel:

Kovács ÁT, Kuipers OP (2013) Transcriptional circuits and phenotypic variation. In: Bacterial Gene Regulation and Transcriptional Networks (Ed. M Madan Babu) Caister Academic Press, Norwich (ISBN: 978-1-908230-14-0)

Kovács KL, Bagi Z, Bálint B, Balogh J, Dávid R, Fodor BD, Csanádi G, Hanczár T, Kovács ÁT, Latinovics D, Maróti G, Mészáros L, Perei K, Tóth A, Rákhely G (2004) Microbial hydrogen metabolism and environmental biotechnology. In: Environmental Biotechnology (Ed. W. Verstraete) Taylor and Francis, London pp. 155-158.

Kovács KL, Bagi Z, Bálint B, Fodor BD, Csanádi G, Csáki R, Hanczár T, Kovács ÁT, Maróti G, Perei K, Tóth A, Rákhely G (2004) Novel approaches to exploit microbial hydrogen metabolism In: Biohydrogen III: Renewable Energy System By Biological Solar Energy Conversion (Ed. J. Miyake, Y Igarashi, M Rogner) Elsevier Science, Amsterdam pp. 13-31.

Zitate: http://scholar.google.com/citations?user=C20-NhAAAAAJ&hl=hu

 

Kontakt

Dr. Ákos T. Kovács
Leiter der Nachwuchsgruppe Terrestrische Biofilme
Institut für Mikrobiologie
Friedrich-Schiller-Universität Jena

Neugasse 23
07743 Jena
Telefon +49 (0)3641 9 49980
https://sites.google.com/site/terrestrialbiofilms/
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