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Vakante Bachelor-, Master- und Diplomarbeiten
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Bachelor- und Masterarbeiten


 

Mikrobielle Kommunikation

 

Prof. Erika Kothe, Dr. Katrin Krause
Ausschreibung für Arbeiten ab 2016-2017

 

Hydrophobins in mycorrhiza formation

Hydrophobins are small proteins, excreted on hyphal tips and covering aerial hyphae. They have functions in protection against drying, colonization of hydrophobic surfaces, and formation of specific tissues. For the ectomycorrhizal fungus Tricholoma vaccinum mycorrhiza-specific and aerial hyphae specific hydrophobins were identified by expression analyses. Hyd4 seems to be involved in the first contact with the root and Hyd5 in mycorrhiza (formation). For further characterization of hydrophobins, overexpression experiments using Agrobacterium tumefaciens mediated transformation should be performed. The successful transformation will be checked by PCR and Southern blot analyses. The validated transformants should be characterized in different treatments to investigate the interaction with other organisms like host specificity with compatible host spruce in comparison with pine, and the tolerance with metal ions.

Ansprechpartnerin: Katrin Krause ( Diese E-Mail-Adresse ist gegen Spambots geschützt! JavaScript muss aktiviert werden, damit sie angezeigt werden kann. )

 

Function of a fungal aldehyde dehydrogenase in plant interaction

Aldehyde dehydrogenases are involved in detoxification mechanisms. They were found to be involved in fungus derived phytohormone production for the mycorrhizal fungus Tricholoma vaccinum affecting symbiosis with the host tree. In phylogenetic analyses, an aldehyde dehydrogenase (protein Id: g2135 in T. vaccinum) is clustering rather with mycorrhizal and phytopathogenic/parasitic than with saprobic gene sequences, which might indicate a function in plant interaction. A search for signal sequences in the promoter/protein sequences should be performed, as well as expression analyses which might help to elucidate the function.

Ansprechpartnerin: Katrin Krause ( Diese E-Mail-Adresse ist gegen Spambots geschützt! JavaScript muss aktiviert werden, damit sie angezeigt werden kann. )

 

Mating type genes in the mycorrhizal fungus Tricholoma vaccinum

Mating interactions and the corresponding mating type genes are fundamental for sexual reproduction of basidiomycete fungi and enable monokaryotic hyphae to mate and form a dikaryotic mycelium, and fruiting bodies with basidiospores. Further, in mycorrhizal fungi fruiting body production depends on the interaction with the plant host. The basidiomycete T. vaccinum forms high compatible interaction with spruce (Picea abies) and low compatible with pine (Pinus sylvestris). Mating type genes will be identified from the fully sequenced genome of T. vaccinum by blast analyses. Their relation to host interaction will be investigated by expression analyses using qRT-PCR with the fungus, mycorrhiza and if available fruiting bodies.

Ansprechpartnerin: Katrin Krause ( Diese E-Mail-Adresse ist gegen Spambots geschützt! JavaScript muss aktiviert werden, damit sie angezeigt werden kann. )

 

Blue colonies as screening tool in Schizophyllum commune

The formation of indigo in S. commune is induced in interactions with other microorganisms. The related genes were identified from the genome sequences in silico. The overexpressing, the elucidation of the inducing signals, and promotor analyses of single genes involved in indigo synthesis could be used to form a nice reporter system easily detectable by blue colonies. Likely, this could be used to test for different stress reactions. The quantification of indigo, the identification of the synthesis and signals inducing indigo synthesis will be part of the project.

Ansprechpartnerin: Katrin Krause ( Diese E-Mail-Adresse ist gegen Spambots geschützt! JavaScript muss aktiviert werden, damit sie angezeigt werden kann. )

 

Investigation of dynein in the white-rot fungus Schizophyllum commune

Dynein is the microtubule-dependent, minus-end directed motor protein, and its heavy chain is encoded of two separate genes in higher basidiomycetes, dhc1 and dhc2. In Schizophyllum commune both genes were deleted in independent experiments to identify their functions in the cell cycle. To further characterize the role of these specific evolutionary two-gene product, Δdhc1 mutant(s) (in a Δku80 background) should be crossed to verify the phenotype in a different genetic background. Crossing of strain Δdhc1 with Δdhc2 should be performed to generate a double mutant. And using a yeast two-hybrid screen, interacting partners should be identified.

Ansprechpartnerin: Katrin Krause ( Diese E-Mail-Adresse ist gegen Spambots geschützt! JavaScript muss aktiviert werden, damit sie angezeigt werden kann. )

 


 

Jena Microbial Resource Collection (JMRC)

 

PD Dr. Kerstin Voigt

Ausschreibung für Masterarbeiten ab 2016-2017

 

1.) Biochemical characterization of the pigment in Lichtheimia corymbifera

Since the turn of the millennium, there has been a massive increase in formerly rare zygomycete infections. Out of a total of 26 species are capable of causing systemic infections. To date little is known about the molecular mechanisms involved in the infection processes of these fungal pathogens. Once manifested in the body the fungal infection – depending on the immune status of the person concerned - leads to death in up to 90 % of all cases. There have been only modest possibilities available with regard to diagnostics and therapy. Our research aims at uncovering the backgrounds of the pathogenity in order to lay a basis for the improvement in diagnostics and new therapeutic possibilities. Interaction analyses of the humanpathogenic mucoralean species Lichtheimia corymbifera revealed a variable virulence potential due to differences in the pigment formation. These are necessary for the adaptation to the environment and play an important role in the interaction with microorganisms. During the course of the project the pigment will be  isolated and biochemically characterized using GC-MS analyses.

Ansprechpartnerin: PD Dr. Kerstin Voigt ( Diese E-Mail-Adresse ist gegen Spambots geschützt! JavaScript muss aktiviert werden, damit sie angezeigt werden kann. )