|2020||Habilitation, venia legendi Microbiology|
|2018 – 2019||Interim Professorship for Microbial Interactions, Friedrich-Schiller-Universität Jena|
|2008 – 2018||Senior Researcher (PI), Applied and Ecological Microbiology, Friedrich-Schiller-Universität Jena|
|2008||Doctoral Thesis, Microbiology, Humboldt-Universität zu Berlin|
|2002||Diploma Biology, Friedrich-Schiller-Universität Jena|
Vitamin B12-containing enzymes fulfill central functions in the energy conservation and cell mass production of anaerobic microbes. The phylogenetically diverse group of reductively dehalogenating bacteria utilizes cobamides (B12 vitamers) as cofactors of the key enzyme in their energy metabolism, the respiratory reductive dehalogenase. This membrane-bound iron-sulfur protein represents the terminal reductase of a respiratory chain that couples the enzymatic reductive dehalogenation of chlorinated or brominated hydrocarbons to the formation of a proton motive force (organohalide respiration).
The research group is studying the composition and functionality of the organohalide respiratory chain in reductively dehalogenating bacteria. Furthermore, the structure and function of reductive dehalogenases are investigated. Unraveling the role of the cobamide cofactor in catalysis, its acquisition and structural variability in different representatives represent a substantial part of our research. We are investigating the mechanism of sensing halogenated organic compounds that triggers the expression of genes involved in organohalide exploitation. The results of our research are going to provide molecular details of a poorly understood mode of anaerobic respiration, which is built on cobamide biochemistry.
Schubert T, von Reuß SH, Kunze C, Paetz C, Brand-Schön P, Mac Nelly A, Nüske J, Diekert G (2019) Guided cobamide biosynthesis for heterologous production of reductive dehalogenases. Microb Biotechnol. 12:346-59.
Keller S, Kunze C, Bommer M, Paetz C, Menezes RC, Svatoš A, Dobbek H, Schubert T (2018) Selective utilization of benzimidazolyl-norcobamides as cofactors by the tetrachloroethene reductive dehalogenase of Sulfurospirillum multivorans. J Bacteriol. 200(8).
Schubert T, Adrian L, Sawers G, Diekert G (2018) Organohalide Respiratory Chains: Composition, Topology, and Key Enzymes. FEMS Microbiol Ecol. 94(4). doi: 10.1093/femsec/fiy035.
Kunze C, Diekert G, Schubert T (2017) Subtle changes in the active site architecture untangled overlapping substrate ranges and mechanistic differences of two reductive dehalogenases. FEBS J 284:3520-35.
Kunze C, Bommer M, Hagen WR, Uksa M, Dobbek H, Schubert T, Diekert G (2017) Cobamide-mediated enzymatic reductive dehalogenation via long-range electron transfer. Nat Commun. 8:15858.
Bommer M, Kunze C, Fesseler J, Schubert T, Diekert G, Dobbek H (2014) Structural basis for organohalide respiration. Science. 346:455-8.
Goris T, Schubert T, Gadkari J, Wubet T, Tarkka M, Buscot F, Adrian L, Diekert G (2014) Insights into organohalide respiration and the versatile catabolism of Sulfurospirillum multivorans gained from comparative genomics and physiological studies. Environ Microbiol. 16:3562-80.
All publications: https://publons.com/researcher/2218856/torsten-schubert/