Fruiting bodies of Schizophyllum commune.

"omics" in the higher basidiomycete Schizophyllum commune

Fruiting bodies of Schizophyllum commune.
Image: Jessica Pötschner

The white rot fungus Schizophyllum commune reproduces via sexual spores only. Thus sexual development is targeted with the mating type loci encoding transcription factors and a pheromone receptor system of 7 transmembrane domain family receptors and their lipopeptide pheromone ligands. The receptors can distinguish between at least 20 different self and non-self pheromones. In response to a non-self pheromone, intracellular, Ras dependent signal transduction leads to induction of dynein dependent nuclear migration. Besides fruiting body development, cell growth and regulation Ras dependent regulation of genes of phosphatidylinositol pathway is investigated, for which a correlation with sexual development is assumed. Mutants of gene thn, originating from the activity of a transposable element and showing a disruption of pheromone receptor gene bbr2 or of RGS protein (regulator of G-protein signaling), are analyzed by mating interactions and the production of volatiles. In addition to sexual development the involvement of S. commune in weathering of Corg-rich rock and wooden material is studied, and the interaction with other fungi and bacteria is investigated. Furthermore lipid rafts should be identified in the fungal membrane, which are proposed to play an important role in the interaction with the environment. Since 2009 the sequenced genome of S. commune is available at www.jgi.doe.gov.

Colleagues in these projects:

Dr. Katrin Krause, Dr. Elke-Martina Jung, Dr. Sophia Wirth, M.Sc. Evans Osahon Iyamu, M.Sc. Nina Carl, M.Sc. Berit Porsche, M.Sc. Jessica Pötschner, M.Sc. Lea Traxler.

Alumni:  Dr. Melanie Brunsch, Dr. Susann Erdmann, Dr. Daniela Freihorst, Dr. Susanne Gola, Dr. Julia Kirtzel, Dr. Nicole Knabe, Dr. Soumya Madhavan, Dr. Riya C. Mezenes, Dr. Reyna Murry, M.Sc. Iman Hardiman, M.Sc. Ülkü Korkmaz, M.Sc. Julia Lindner, Dipl.-Biol. Daniela Schubert, M.Sc. Susanne Steiniger.

Most important publications:

Murry R, Pötschner J, Traxler L, Krüger T, Kniemeyer O, Krause K, Kothe E. (2021) Inositol signaling in the basidiomycete fungus Schizophyllum commune. Journal of Fungi 7, 470. https://doi.org/10.3390/jof7060470.

Wirth S, Freihorst D, Krause K, Kothe E (2021) What role might non-mating receptors play in Schizophyllum commune? Journal of Fungi 7(5):399. https://doi.org/10.3390/jof7050399.

Traxler L, Wollenberg A, Steinhauser G, Chyzhevskyi I, Dubchak S, Grossmann S, Gunther A, Gupta DK, Iwannek KH, Kirieiev S, Lehmann F, Schulz W, Walther C, Raff J, Kothe, E (2021) Survival of the basidiomycete Schizophyllum commune in soil under hostile environmental conditions in the Chernobyl Exclusion Zone Journal of Hazardous Materials 403, 124002 DOI: 10.1016/j.jhazmat.2020.124002.

Wirth S, Krause K, Kunert M, Broska S, Paetz C, Boland W, Kothe E (2021). Function of sesquiterpenes from Schizophyllum commune in interspecific interactions. PLoS ONE 16(1): e0245623. https://doi.org/10.1371/journal.pone.0245623.

Krause K, Jung E-M, Lindner J, Hardiman I, Poetschner J, Madhavan S, Matthäus C, Kai M, Menezes RC, Popp J, Svatoš A, Kothe E. (2020). Response of the white rot fungus Schizophyllum commune to co-occurring microorganisms. PLoS ONE 15(4): e0232145. https://doi.org/10.1371/journal.pone.0232145.

Murry R, Kniemeyer O, Krause K, Saiardi A, Kothe E (2019). Crosstalk between Ras and inositol phosphate signaling revealed by lithium action on inositol monophosphatase in Schizophyllum commune, Adv Biol Regul 72, 78-88. 10.1016/j.jbior.2019.01.001.

Kirtzel J, Ueberschaar N, Deckert-Gaudig T, Krause K, Deckert V, Gadd GM, Kothe E. (2019). Organic acids, siderophores, enzymes and mechanical pressure for black slate bioweathering with the basidiomycete Schizophyllum commune. Environ Microbiol doi:10.1111/1462-2920.14749.

Jung EM, Kothe E, Raudaskoski M. (2018). The making of a mushroom: Mitosis, nuclear migration and the actin network. Fungal Genet Biol 111, 85-91. doi: 10.1016/j.fgb.2017.11.001.

Kirtzel J, Madhavan S, Wielsch N, Blinne A, Hupfer Y, Linde J, Krause K, Svatoš A and Kothe E (2018). Enzymatic bioweathering and metal mobilization from black slate by the basidiomycete Schizophyllum commune. Front Microbiol 9, 2545. doi: 10.3389/fmicb.2018.02545.

Kirtzel J, Scherwietes EL, Merten D, Krause K, Kothe E (2018). Metal release and sequestration from black slate mediated by a laccase of Schizophyllum commune. Environ Sci Pollut Res doi: 10.1007/s11356-018-2568-z [Epub ahead of print].

Wirth S, Kunert M, Ahrens L-M, Krause K, Broska S, Paetz C, Kniemeyer O, Jung E-M, Boland W, Kothe.E (2018). The regulator of G-protein signaling Thn1 links pheromone response to volatile production in Schizophyllum commune. Environ Microbiol 20, 3684-3699.

Freihorst D, Brunsch M, Wirth S, Krause K, Kniemeyer O, Linde J, Kunert M, Boland W, Kothe E (2016). Smelling the difference: Regulating transcriptome, proteome and volatilome changes after mating. Fungal Genet Biol. FGB-16-154R2. DOI: 10.1016/j.fgb.2016.08.007.

Brunsch M, Schubert D, Gube M, Ring C, Hanisch L, Linde J, Krause K, Kothe E. (2015). Dynein heavy chain, encoded by two genes in agaricomycetes, is required for nuclear migration in Schizophyllum commune. PLoS One e0135616.

Menezes RC, Kai M, Krause K, Matthäus C, Svatoš A, Popp J, Kothe E. (2015). Monitoring metabolites from Schizophyllum commune interacting with Hypholoma fasciculare combining LESA-HR mass spectrometry and Raman microscopy. Anal Bioanal Chem 407, 2273-2282.

Freihorst D, Fowler TJ, Bartholomew K, Raudaskoski M, Horton S, Kothe E. (2015). The mating type genes of the basidioymcetes. In: Wendland J, Esser K (eds) The Mycota. Springer, Heidelberg. In press.

Madhavan S, Krause K, Jung E-M, Kothe E. (2014). Differential regulation of multi-copper oxidases in Schizophyllum commune during sexual development Mycol Progr 13, 1199-1206.

Knabe N, Jung E-M, Freihorst D, Hennicke F, Horton S, Kothe E. (2013). A central role for Ras1 in morphogenesis of the basidiomycete Schizophyllum commune. Euk Cell 12, 941-952.